Journal: bioRxiv

Article Title: NDNF+ interneurons have a privileged role in regulating cortical excitability

doi: 10.1101/2025.11.27.690945

Figure Lengend Snippet: ( A ) mCherry expression in NDNF+ neurons within the stratum lacunosum moleculare (sl-m) of the hippocampus from mice injected with either intrahippocampal saline (control) or intrahippocampal kainate (epileptic). DG = dentate gyrus. The number of mCherry-positive cells per area was unchanged in epileptic animals (the unpaired mean difference between Saline and Kainate is 26,3% [95%CI, 7.8, 50.5], p=0.072 two-sided permutation t-test, and p= 0.06 Student’s t-test, Saline n = 8 slices /3 mice; Kainate n= 20 slices /3 mice). ( B ) Experimental setup. ( C ) Representative LFP traces from hippocampal depth electrodes from a mouse expressing hM3Dq in NDNF+ neurons before and after intraperitoneal (i.p) injection of either DMSO (0.1% in saline) control or DCZ (10 μg/kg).

Article Snippet: In the meantime, an LFP recording electrode (borosilicate glass pipettes 1.5 mm OD, 0.86 mm ID Warner Instruments, G150F-4 with a resistance of 2-3 MΩ) filled with saline was positioned at the centre of the craniotomy above the pilocarpine injection site and lowered to 0.3 mm below the pia, and a bare ended optic fibre (ø: 200 μm, NA: 0.22, Multimode 190–1200 nm, Thorlabs Inc., UK) was positioned 1.5-2 mm above the pia ( Suppl.

Techniques: Expressing, Injection, Saline, Control